Validated methods for the quantification of biologically active constituents of poplar-type propolis.
Identifieur interne : 004133 ( Main/Exploration ); précédent : 004132; suivant : 004134Validated methods for the quantification of biologically active constituents of poplar-type propolis.
Auteurs : Milena Popova [Bulgarie] ; Vassya Bankova ; Daniela Butovska ; Valentin Petkov ; Boryana Nikolova-Damyanova ; Anna Gloria Sabatini ; Gian Luigi Marcazzan ; Stefan BogdanovSource :
- Phytochemical analysis : PCA [ 0958-0344 ]
Descripteurs français
- KwdFr :
- MESH :
- analyse : Flavanones, Flavonoïdes, Phénols.
- composition chimique : Anti-infectieux, Propolis.
- métabolisme : Populus.
- méthodes : Chromatographie en phase liquide à haute performance, Spectrophotométrie.
- Reproductibilité des résultats.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Flavanones, Flavonoids, Phenols.
- chemical , chemistry : Anti-Infective Agents, Propolis.
- metabolism : Populus.
- methods : Chromatography, High Pressure Liquid, Spectrophotometry.
- Reproducibility of Results.
Abstract
The validation of rapid, low-cost spectrophotometric procedures for the quantification of the three main groups of bioactive substances (flavones and flavonols, flavanones and dihydroflavonols, and total phenolics) in poplar-type propolis has been performed. A spectrophotometric assay based on the formation of an aluminium chloride complex was applied for the quantification of total flavones and flavonols using galangin as standard. Because of the high amount of flavanones and dihydroflavonols in "poplar type" propolis, the introduction of a distinct procedure for their quantification was considered of special significance and the DAB9 colorimetric method was applied for the purpose. Total phenolic content was measured by the Folin-Ciocalteu procedure using a mixture of pinocembrin and galangin as a reference. The procedures were validated using a model mixture of compounds representing the poplar-type propolis composition as found in previous studies. The accuracy (recovery) varied in the range 84-109%, and the relative standard deviation was 0.5-6.2%. The developed spectrophotometric procedures were applied to six poplar type propolis samples. The results were verified independently by a HPLC procedure. The two sets of results agreed satisfactory, as proven by Student's t-test.
DOI: 10.1002/pca.777
PubMed: 15311843
Affiliations:
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uniqKey="Butovska D" first="Daniela" last="Butovska">Daniela Butovska</name></author><author><name sortKey="Petkov, Valentin" sort="Petkov, Valentin" uniqKey="Petkov V" first="Valentin" last="Petkov">Valentin Petkov</name></author><author><name sortKey="Nikolova Damyanova, Boryana" sort="Nikolova Damyanova, Boryana" uniqKey="Nikolova Damyanova B" first="Boryana" last="Nikolova-Damyanova">Boryana Nikolova-Damyanova</name></author><author><name sortKey="Sabatini, Anna Gloria" sort="Sabatini, Anna Gloria" uniqKey="Sabatini A" first="Anna Gloria" last="Sabatini">Anna Gloria Sabatini</name></author><author><name sortKey="Marcazzan, Gian Luigi" sort="Marcazzan, Gian Luigi" uniqKey="Marcazzan G" first="Gian Luigi" last="Marcazzan">Gian Luigi Marcazzan</name></author><author><name sortKey="Bogdanov, Stefan" sort="Bogdanov, Stefan" uniqKey="Bogdanov S" first="Stefan" last="Bogdanov">Stefan Bogdanov</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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xml:lang="fr">Bulgarie</country><wicri:regionArea>Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Bankova, Vassya" sort="Bankova, Vassya" uniqKey="Bankova V" first="Vassya" last="Bankova">Vassya Bankova</name></author><author><name sortKey="Butovska, Daniela" sort="Butovska, Daniela" uniqKey="Butovska D" first="Daniela" last="Butovska">Daniela Butovska</name></author><author><name sortKey="Petkov, Valentin" sort="Petkov, Valentin" uniqKey="Petkov V" first="Valentin" last="Petkov">Valentin Petkov</name></author><author><name sortKey="Nikolova Damyanova, Boryana" sort="Nikolova Damyanova, Boryana" uniqKey="Nikolova Damyanova B" first="Boryana" last="Nikolova-Damyanova">Boryana Nikolova-Damyanova</name></author><author><name sortKey="Sabatini, Anna Gloria" sort="Sabatini, Anna Gloria" uniqKey="Sabatini A" first="Anna Gloria" last="Sabatini">Anna Gloria Sabatini</name></author><author><name sortKey="Marcazzan, Gian Luigi" sort="Marcazzan, Gian Luigi" uniqKey="Marcazzan G" first="Gian Luigi" last="Marcazzan">Gian Luigi Marcazzan</name></author><author><name sortKey="Bogdanov, Stefan" sort="Bogdanov, Stefan" uniqKey="Bogdanov S" first="Stefan" last="Bogdanov">Stefan Bogdanov</name></author></analytic><series><title level="j">Phytochemical analysis : PCA</title><idno type="ISSN">0958-0344</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Infective Agents (chemistry)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Flavanones (analysis)</term><term>Flavonoids (analysis)</term><term>Phenols (analysis)</term><term>Populus (metabolism)</term><term>Propolis (chemistry)</term><term>Reproducibility of Results (MeSH)</term><term>Spectrophotometry (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anti-infectieux (composition chimique)</term><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Flavanones (analyse)</term><term>Flavonoïdes (analyse)</term><term>Phénols (analyse)</term><term>Populus (métabolisme)</term><term>Propolis (composition chimique)</term><term>Reproductibilité des résultats (MeSH)</term><term>Spectrophotométrie (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Flavanones</term><term>Flavonoids</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anti-Infective Agents</term><term>Propolis</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Flavanones</term><term>Flavonoïdes</term><term>Phénols</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Anti-infectieux</term><term>Propolis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrophotometry</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Spectrophotométrie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Reproductibilité des résultats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The validation of rapid, low-cost spectrophotometric procedures for the quantification of the three main groups of bioactive substances (flavones and flavonols, flavanones and dihydroflavonols, and total phenolics) in poplar-type propolis has been performed. A spectrophotometric assay based on the formation of an aluminium chloride complex was applied for the quantification of total flavones and flavonols using galangin as standard. Because of the high amount of flavanones and dihydroflavonols in "poplar type" propolis, the introduction of a distinct procedure for their quantification was considered of special significance and the DAB9 colorimetric method was applied for the purpose. Total phenolic content was measured by the Folin-Ciocalteu procedure using a mixture of pinocembrin and galangin as a reference. The procedures were validated using a model mixture of compounds representing the poplar-type propolis composition as found in previous studies. The accuracy (recovery) varied in the range 84-109%, and the relative standard deviation was 0.5-6.2%. The developed spectrophotometric procedures were applied to six poplar type propolis samples. The results were verified independently by a HPLC procedure. The two sets of results agreed satisfactory, as proven by Student's t-test.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15311843</PMID><DateCompleted><Year>2004</Year><Month>09</Month><Day>21</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0958-0344</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>4</Issue><PubDate><MedlineDate>2004 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>Phytochemical analysis : PCA</Title><ISOAbbreviation>Phytochem Anal</ISOAbbreviation></Journal><ArticleTitle>Validated methods for the quantification of biologically active constituents of poplar-type propolis.</ArticleTitle><Pagination><MedlinePgn>235-40</MedlinePgn></Pagination><Abstract><AbstractText>The validation of rapid, low-cost spectrophotometric procedures for the quantification of the three main groups of bioactive substances (flavones and flavonols, flavanones and dihydroflavonols, and total phenolics) in poplar-type propolis has been performed. A spectrophotometric assay based on the formation of an aluminium chloride complex was applied for the quantification of total flavones and flavonols using galangin as standard. Because of the high amount of flavanones and dihydroflavonols in "poplar type" propolis, the introduction of a distinct procedure for their quantification was considered of special significance and the DAB9 colorimetric method was applied for the purpose. Total phenolic content was measured by the Folin-Ciocalteu procedure using a mixture of pinocembrin and galangin as a reference. The procedures were validated using a model mixture of compounds representing the poplar-type propolis composition as found in previous studies. The accuracy (recovery) varied in the range 84-109%, and the relative standard deviation was 0.5-6.2%. The developed spectrophotometric procedures were applied to six poplar type propolis samples. The results were verified independently by a HPLC procedure. The two sets of results agreed satisfactory, as proven by Student's t-test.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Popova</LastName><ForeName>Milena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bankova</LastName><ForeName>Vassya</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Butovska</LastName><ForeName>Daniela</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Petkov</LastName><ForeName>Valentin</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Nikolova-Damyanova</LastName><ForeName>Boryana</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Sabatini</LastName><ForeName>Anna Gloria</ForeName><Initials>AG</Initials></Author><Author ValidYN="Y"><LastName>Marcazzan</LastName><ForeName>Gian Luigi</ForeName><Initials>GL</Initials></Author><Author ValidYN="Y"><LastName>Bogdanov</LastName><ForeName>Stefan</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochem Anal</MedlineTA><NlmUniqueID>9200492</NlmUniqueID><ISSNLinking>0958-0344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D044950">Flavanones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005419">Flavonoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>9009-62-5</RegistryNumber><NameOfSubstance UI="D011429">Propolis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="N">Anti-Infective Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044950" MajorTopicYN="N">Flavanones</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005419" MajorTopicYN="N">Flavonoids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011429" MajorTopicYN="N">Propolis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013053" MajorTopicYN="N">Spectrophotometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>8</Month><Day>18</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>9</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>8</Month><Day>18</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15311843</ArticleId><ArticleId IdType="doi">10.1002/pca.777</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Bulgarie</li></country><region><li>Sofia-ville (oblast)</li></region><settlement><li>Sofia</li></settlement></list><tree><noCountry><name sortKey="Bankova, Vassya" sort="Bankova, Vassya" uniqKey="Bankova V" first="Vassya" last="Bankova">Vassya Bankova</name><name sortKey="Bogdanov, Stefan" sort="Bogdanov, Stefan" uniqKey="Bogdanov S" first="Stefan" last="Bogdanov">Stefan Bogdanov</name><name sortKey="Butovska, Daniela" sort="Butovska, Daniela" uniqKey="Butovska D" first="Daniela" last="Butovska">Daniela Butovska</name><name sortKey="Marcazzan, Gian Luigi" sort="Marcazzan, Gian Luigi" uniqKey="Marcazzan G" first="Gian Luigi" last="Marcazzan">Gian Luigi Marcazzan</name><name sortKey="Nikolova Damyanova, Boryana" sort="Nikolova Damyanova, Boryana" uniqKey="Nikolova Damyanova B" first="Boryana" last="Nikolova-Damyanova">Boryana Nikolova-Damyanova</name><name sortKey="Petkov, Valentin" sort="Petkov, Valentin" uniqKey="Petkov V" first="Valentin" last="Petkov">Valentin Petkov</name><name sortKey="Sabatini, Anna Gloria" sort="Sabatini, Anna Gloria" uniqKey="Sabatini A" first="Anna Gloria" last="Sabatini">Anna Gloria Sabatini</name></noCountry><country name="Bulgarie"><region name="Sofia-ville (oblast)"><name sortKey="Popova, Milena" sort="Popova, Milena" uniqKey="Popova M" first="Milena" last="Popova">Milena 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